Your Position: Home > Support > Antibody Validation > New Phospho-specificity Verification with Phosphatase
Antibody Validation

New Phospho-specificity Verification with Phosphatase

In order to further verify phospho-specificity and ensure quality, SAB has increased phosphatase treatment method in validation of phospho-specific antibodies. Up to the present SAB has used numerous analytical methods in antibody characterization.
         Western blotting(WB)
                  Phosphatase treatment
                  Stimulus treatment
                  Blocking peptide control
                  Loading control
                  Validation by various cell lines
         Immunohistochemistry(IHC)
                  Blocking peptide control
                  Validation with different tissues
         Immunofluorescence / Immunocytochemistry(IF/ICC)
                 Validation by various cell lines
                 Subcellular localization and co-localization analysis
         Flow cytometry(FCM)
  
         ELISA

 

Akt(Phospho-Ser473) Antibody 11054
Western blot analysis of extracts from 293 cells, treatedwith EGF or calf intestinal phosphatase (CIP), using Akt(Phospho-Ser473) Antibody #11054.
MORE>

 

PTEN(Phospho-Ser380/Thr382/Thr383) Antibody 11056
Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody #11056.
MORE>

 

Tau(Phospho-Ser404) Antibody 11112
Western blot analysis of extracts from C6 cells, treated with calf intestinal phosphatase (CIP), using Tau (Phospho-Ser404) Antibody #11112.
MORE>

 

NFkB-p65(Phospho-Ser529) Antibody 11217
Western blot analysis of extracts from MCF cells, treated with TNFa+CA or calf intestinal phosphatase (CIP), using NFκB-p65 (Phospho-Ser529) Antibody #11217.
MORE>

 

mTOR(Phospho-Ser2448) Antibody 11221
Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using mTOR (Phospho-Ser2448) Antibody #11221.
MORE>

 

1 2 3
    SUBSCRIBE OUR E-MAIL
  • Inspiration, ideas and your feedback.
FOLLOW US

facebook twitter linkedin

sab © 2017 Signalway Antibody LLC. All Rights Reserved.  E-mail: info@signalwayantibody.com