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Antibody Validation

Immunohistochemistry(IHC)

 

Immunohistochemistry (IHC) is commonly used for in situ qualitative or quantitative analysis of a target protein in cells of a tissue section. Thought a labeled antibody that is specific to its antigen and sensitive to color-developing agent, this method is also widely used for analyzing the sub-cellular localization of a protein. SAB offers IHC applicable antibodies which have generally been validated by different tissues and various approaches. This will distinctly save your time in experiment and can avoid waste of samples.

 
Validation Approaches Include:
      Validation by different tissues
      Blocking peptide control
     
Human breast carcinoma tissue
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκB-p65 (Phospho-Ser536) Antibody #11014.
 
Human Lung carcinoma tissue
Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using NFκB-p65 (Phospho-Ser536) Antibody #11014.

 

Human colon carcinoma tissue

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using IKK α (Phospho-Thr23) Antibody #11129.
 
Human tonsil carcinoma tissue
Immunohistochemical analysis of paraffin-embedded human tonsil carcinoma tissue using VASP (Phospho-Ser157) Antibody #11214
 
Rat hippocampal region tissue
Immunohistochemical analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer’s Disease using Tau (Phospho-Thr205) Antibody #11108.
 
Blocking peptide control
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p56Dok-2 (Phospho-Tyr299) Antibody #11278 (left) or the same antibody preincubated with blocking peptide #51278 (right).
 

 

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