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Antibody Validation

Immunofluorescence (IF)

 

Immunofluorescence (IF) is used to analyze the sub-cellular localization of a protein within a cell. This technique usually uses a labeled antibody to target the fluorescent dye to its antigen. For the emitted light by the fluorescent dye can be detected in fluorescence microscope, this allows visualization of the distribution of a target protein in the sample. SAB follows stringent validation protocol to ensure IF application.   

 
Immunofluorescence (IF) validation methods:
      Using appropriate cell lines for analysis
      Stimulus treatment
      Inhibitor treatment
 
Using appropriate cell lines for analysis
Immunofluorescence analysis of methanol-fixed MCF7 cells using HER2 (Phospho-Tyr1248) Antibody #11079.
 
Immunofluorescence analysis of methanol-fixed Hela cells using GSK3β (Phospho-Ser9) Antibody #11002.
 
 
 Immunofluorescence analysis of methanol-fixed MEF cells using Histone H3 (Tri-Methyl-Lys27) Antibody #11582.
 
Stimulus treatment
Immunofluorescence analysis of methanol-fixed MEF cells untreated or treated with IFN using STAT3 (Phospho-Tyr705) Antibody #11045.

 

Inhibitor treatment

Immunofluorescence staining of methanol-fixed MEF cells untreated or treated with LY2904 using FKHR (Phospho-Ser256) Antibody #11115.

 
 

 

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