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His-Tag Mouse Monoclonal Antibody (4E6)

T523
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  Catalog No. Package Size Price
T523 100ul $279.00

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Description
  • Product Name His-Tag Mouse Monoclonal Antibody (4E6)
  • Host Species Mouse
  • Clonality Monoclonal
  • Clone No. 4E6
  • Purification Affinity purification using immunogen.
  • Applications WB
  • Specificity The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins..
  • Target Name His-Tag
  • Concentration 1.0mg/ml
  • Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:3000~1:10000

Images
  • His-Tag Mouse Monoclonal Antibody (4E6) - SAB | Signalway Antibody

    Western blot analysis of His recombinant protein, using #T523 diluted at 1£©1:10,000, 2£©1:20,000

Background

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.

    Please let us know if you have published research using #T523 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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