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Flag-tag Mouse Monoclonal Antibody (2C5)

T524
Review: comment rank 5 pdf
  Catalog No. Package Size Price
T524 100ul $279.00

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Description
  • Product Name Flag-tag Mouse Monoclonal Antibody (2C5)
  • Host Species Mouse
  • Clonality Monoclonal
  • Clone No. 2C5
  • Purification Affinity purification using immunogen.
  • Applications WB
  • Specificity The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion?proteins.
  • Target Name Flag-tag
  • Concentration 1.0mg/ml
  • Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:5000~1:10000

Images
  • Flag-tag Mouse Monoclonal Antibody (2C5) - SAB | Signalway Antibody

    Western blot analysis of Recombinant protein, using #T524 diluted at 1) 1:5,000, 2) 1:10,000.

Background

The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.

    Please let us know if you have published research using #T524 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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