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HA-tag Mouse Monoclonal Antibody (1B10)

T525
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  Catalog No. Package Size Price
T525 100ul $279.00

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Description
  • Product Name HA-tag Mouse Monoclonal Antibody (1B10)
  • Host Species Mouse
  • Clonality Monoclonal
  • Clone No. 1B10
  • Purification Affinity purification using immunogen.
  • Applications WB
  • Specificity The HA?tag antibody can recognize C-terminal, internal, and N-terminal HA-tag fusion?proteins.
  • Target Name HA-tag
  • Concentration 1.0mg/ml
  • Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:1000~1:3000

Images
  • HA-tag Mouse Monoclonal Antibody (1B10) - SAB | Signalway Antibody

    Western blot analysis of HA Recombinant protein, using #T525 diluted at 1:2,000.

Background

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.HA tag antibody is a highly sensitive and affinity monoclonal antibody applicable to HA-tagged fusion protein detection.

    Please let us know if you have published research using #T525 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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