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α-tubulin Mouse Monoclonal Antibody

37981
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  Catalog No. Package Size Price
37981 100ul $279.00

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Description
  • Product Name α-tubulin Mouse Monoclonal Antibody
  • Host Species Mouse
  • Clonality Monoclonal
  • Purification Affinity purification using immunogen.
  • Applications WB IF IP
  • Species Reactivity Hu Rt Ms
  • Specificity The α-tubulin antibody can detects endogenousα-tubulin protein.
  • Target Name α-tubulin
  • Alternative Names alpha-tubulin N-acetyltransferase; ATAT1; C6orf134; CF134;
  • Accession No. Swiss-Prot#:Q71U36/P68363 Gene ID 7846/10376
  • SDS-PAGE MW 52kd
  • Concentration 1.0mg/ml
  • Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:5000~1:10000
Immunofluorescence: 1:100~1:200
Immunoprecipitation: 1:200

Images
  • α-tubulin Mouse Monoclonal Antibody - SAB | Signalway Antibody

    Western blot analysis of 1) Hela, 2) Rat BrianTissue, 3) Mouse Brain Tissue, using #37981 diluted at 1:5,000.

  • α-tubulin Mouse Monoclonal Antibody - SAB | Signalway Antibody

    IF analysis of?Hela?with #37981(Left) and DAPI (Right) diluted at 1:100.

  • α-tubulin Mouse Monoclonal Antibody - SAB | Signalway Antibody

    1¡¢Input: Mouse Brain Tissue Lysate
    2¡¢IP product: IP dilute 1:200
    Western blot analysis: primary antibody : #37981 1:5,000
    Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1:5,000

Background

Tubulin is one of several members of a small family of globular proteins. The tubulin superfamily includes five distinct families, the alpha-, beta-, gamma-, delta-, and epsilon-tubulins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 KD. Microtubules are assembled from dimers of α- and β-tubulin

    Please let us know if you have published research using #37981 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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