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GAPDH Rabbit Polyclonal Antibody

37985
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  Catalog No. Package Size Price
37985 100ul $279.00

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Description
  • Product Name GAPDH Rabbit Polyclonal Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Affinity purification using immunogen.
  • Applications WB IHC
  • Species Reactivity Hu Rt Ms Mk Chk Hm Sh Xenopus
  • Specificity Antibody detects endogenous GAPDH protein.
  • Target Name GAPDH
  • Alternative Names G3PD; GAPD; MGC88685
  • Accession No. Swiss-Prot#:P04406
  • SDS-PAGE MW 37kd
  • Concentration 1.0mg/ml
  • Formulation IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:5000~1:20000

Images
  • GAPDH Rabbit Polyclonal Antibody - SAB | Signalway Antibody

    Western blot analysis of 293T (1), Rat brain (2), NIH 3T3(3), Sheep Muscle(4),Rabbit testis(5), using #37985 diluted at 1:20,000.

Background

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

    Please let us know if you have published research using #37985 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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