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β-tubulin Mouse Monoclonal Antibody(Zebrafish Specific)

38058
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  Catalog No. Package Size Price
38058 100ul $279.00

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Description
  • Product Name β-tubulin Mouse Monoclonal Antibody(Zebrafish Specific)
  • Host Species Mouse
  • Clonality Monoclonal
  • Purification Affinity purification using immunogen.
  • Applications WB IHC IF
  • Species Reactivity Zebrafish
  • Specificity Theβ-tubulin antibody can detects Zebrafish endogenousβ-tubulin protein.
  • Target Name β-tubulin
  • Alternative Names beta 5-tubulin; beta Ib tubulin; beta-4 tubulin; M40; MGC117247; MGC16435; OK/SW-cl.56; TBB5; TUBB; TUBB1; TUBB5
  • Accession No. Swiss-Prot#:P07437
  • SDS-PAGE MW 55kd
  • Concentration 1.0mg/ml
  • Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20˚C
Application Details

Western blotting: 1:5000~1:20000

Images
  • β-tubulin Mouse Monoclonal Antibody(Zebrafish Specific) - SAB | Signalway Antibody

    Western blot analysis of Zebrafish skeletal muscle, using #38058 diluted at 1:5,000.

Background
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.
    Please let us know if you have published research using #38058 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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