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p53(Phospho-Ser315) Antibody

11100
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  Catalog No. Package Size Price
11100-1 50ul $189.00
11100-2 100ul $279.00

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Description
  • Product Name p53(Phospho-Ser315) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC IF
  • Species Reactivity Hu
  • Specificity The antibody detects endogenous level of p53 only when phosphorylated at serine 315.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of serine 315 (S-S-S(p)-P-Q) derived from Human p53.
  • Target Name p53
  • Modification Phospho
  • Alternative Names Tumor suppressor p53; Phosphoprotein p53; Antigen NY-CO-13; TP53;
  • Accession No. Swiss-Prot: P04637
    NCBI Protein: NP_000537.3
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 53kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images
  • p53(Phospho-Ser315) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from Hela cells using p53(Phospho-Ser315) Antibody #11100(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).

  • p53(Phospho-Ser315) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53(Phospho-Ser315) Antibody #11100(left) or the same antibody preincubated with blocking peptide(right).

  • p53(Phospho-Ser315) Antibody - SAB | Signalway Antibody

    Immunofluorescence staining of methanol-fixed Hela cells using p53(Phospho-Ser315) Antibody #11100.

Background
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.

Lu, H. et al. (1997) Mol. Cell. Biol. 17, 5923-5934.
Lohrum, M. et.al. (1996) Oncogene 13, 2527-2539.
Posp
    Please let us know if you have published research using #11100 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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