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FKHR(Phospho-Ser256) Antibody

11115
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  Catalog No. Package Size Price
11115-1 50ul $189.00
11115-2 100ul $279.00

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Description
  • Product Name FKHR(Phospho-Ser256) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC IF IP
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of FKHR only when phosphorylated at serine 256.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of serine 256 (A-A-S(p)-M-D) derived from Human FKHR.
  • Target Name FKHR
  • Modification Phospho
  • Alternative Names FKHR; FOXO1;
  • Accession No. Swiss-Prot: Q12778
    NCBI Protein: NP_002006.2
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 78-82 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images
  • FKHR(Phospho-Ser256) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from 293 cells untreated or treated with serum using FKHR(Phospho-Ser256) Antibody #11115.

  • FKHR(Phospho-Ser256) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHR (Phospho-Ser256) Antibody #11115 (left) or the same antibody preincubated with blocking peptide #51115 (right).

  • FKHR(Phospho-Ser256) Antibody - SAB | Signalway Antibody

    Immunofluorescence staining of methanol-fixed MCF7 cells using FKHR(Phospho-Ser256) Antibody #11115.

  • FKHR(Phospho-Ser256) Antibody - SAB | Signalway Antibody

    Immunofluorescence staining of methanol-fixed MEF cells untreated or treated with LY2904 using FKHR (Phospho-Ser256) Antibody #11115.

  • FKHR(Phospho-Ser256) Antibody - SAB | Signalway Antibody

    Immunoprecipitation analysis of extracts of 293T cells incubated with FKHR (Phospho-Ser256) Antibody

Background

Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts syngernistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A to activate the expression of genes such as IGFBP1, G6PC and PPCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1. Promotes neural cell death. Mediates insulin action on adipose. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells.

Guo S., Rena G., Cichy S., He X., Cohen P., Unterman T.J. Biol. Chem. 274:17184-17192(1999)              
Zhang X., Gan L., Pan H., Guo S., He X., Olson S.T., Mesecar A., Adam S., Unterman T.G.J. Biol. Chem. 277:45276-45284(2002)              
Daitoku H., Hatta M., Matsuzaki H., Aratani S., Ohshima T., Miyagishi M., Nakajima T., Fukamizu A.Proc. Natl. Acad. Sci. U.S.A. 101:10042-10047(2004)
  • Zhan Lixuan, Tao Wang, Wen Li et al,Activation of AKT/FoxO signaling pathway contributes to induction of neuroprotection against transient global cerebral ischemia by hipoxic pre-conditioning in adult rats., Journal of Neurochemistry, 114(3):897-908 (2010),
    PMID: 20492357
  • Please let us know if you have published research using #11115 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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