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LIMK1(Phospho-Thr508) Antibody

11126
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  Catalog No. Package Size Price
11126-1 50ul $189.00
11126-2 100ul $279.00

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Description
  • Product Name LIMK1(Phospho-Thr508) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC IF
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of LIMK1 only when phosphorylated at threonine 508.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of threonine 508 (R-Y-T(p)-V-V) derived from Human LIMK1.
  • Target Name LIMK1
  • Modification Phospho
  • Alternative Names LIMK-1; kinase LIMK1;
  • Accession No. Swiss-Prot: P53667
    NCBI Protein: NP_002305.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 72kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images
  • LIMK1(Phospho-Thr508) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from UV-treated C2C12, PMA-treated Hela and anisomycin-treated 293 cells using LIMK1(Phospho-Thr508) Antibody #11126.

  • LIMK1(Phospho-Thr508) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK1(Phospho-Thr508) Antibody #11126(left) or the same antibody preincubated with blocking peptide(right).

  • LIMK1(Phospho-Thr508) Antibody - SAB | Signalway Antibody

    Immunofluorescence staining of methanol-fixed Hela cells using LIMK1(Phospho-Thr508) Antibody #11126.

  • LIMK1(Phospho-Thr508) Antibody - SAB | Signalway Antibody

    Lane 1 : Hela treated with PMA; Lane 2 : Mouse brain tissue; Lane 3 : Rat brain tissue; Lane 4 : N-peptide blocked Hela treated with PMA; Lane 5 : N-peptide blocked Mouse brain tissue; Lane 6 : N-peptide blocked Rat brain tissue; Lane 7 : P-peptide blocked Hela treated with PMA; Lane 8 : P-peptide blocked Mouse brain tissue; Lane 9 : P-peptide blocked Rat brain tissue; Lysates/proteins at 40 µg per lane. Predicted band size : 72 kDa Observed band size : 72 kDa

Background

Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development.

Soosairajah J, et al. (2005) EMBO J.
Ohashi K, et al. (2000) J Biol Chem; 275(5): 3577-82.
Edwards DC, et al. (1999) Nat Cell Biol; 1(5): 253-9.

  • Li, X., Ke, Q., Li, Y. et al,DGCR6L, A Novel PAK4 Interaction Protein, Regulates PAK4-mediated migration of Human Gastric Cancer Cell via LIMK1., International Journal of Biochemistry and Cell Biology, 42: 70¨C79 (2008),
    PMID: 19778628
  • Please let us know if you have published research using #11126 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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