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cofilin(Phospho-Ser3) Antibody

11139
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  Catalog No. Package Size Price
11139-1 50ul $189.00
11139-2 100ul $279.00

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Description
  • Product Name cofilin(Phospho-Ser3) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC IF
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of cofilin only when phosphorylated at serine 3.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of serine 3 (M-A-S(p)-G-V) derived from Human cofilin.
  • Target Name cofilin
  • Modification Phospho
  • Alternative Names CFL; CFL1;
  • Accession No. Swiss-Prot: P23528
    NCBI Protein: NP_005498.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 19kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images
  • cofilin(Phospho-Ser3) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from COLO205 cells using cofilin(Phospho-Ser3) Antibody #11139(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).

  • cofilin(Phospho-Ser3) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin(Phospho-Ser3) Antibody #11139(left) or the same antibody preincubated with blocking peptide(right).

  • cofilin(Phospho-Ser3) Antibody - SAB | Signalway Antibody

    Immunofluorescence staining of methanol-fixed Hela cells using cofilin(Phospho-Ser3) Antibody #11139.

  • cofilin(Phospho-Ser3) Antibody - SAB | Signalway Antibody

    Lane 1 : Hela treated with H2O2; Lane 2 : Mouse brain tissue; Lane 3 : Rat brain tissue; Lane 4 : N-peptide blocked Hela treated with H2O2; Lane 5 : N-peptide blocked Mouse brain tissue; Lane 6 : N-peptide blocked Rat brain tissue; Lane 7 : P-peptide blocked Hela treated with H2O2; Lane 8 : P-peptide blocked Mouse brain tissue; Lane 9 : P-peptide blocked Rat brain tissue; Lysates/proteins at 40 µg per lane. Predicted band size : 19 kDa Observed band size : 19 kDa

Background

Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

Kobayashi M, et al. (2006) EMBO J 25(4): 713-26.
Wang Y, et al. (2005) Biol Chem 280(13): 12683-9.
Smith-Beckerman DM, et al. (2005) Mol Cell Proteomics: 156-68.

  • Guillaume Huet1, Eeva Kaisa Rajakyla, Tiina Viita1 et al,Actin-regulated feedback loop based on Phactr4, PP1 and cofilin maintains the actin monomer pool., Journal of Cell Science, 126:497¨C507 (2012),
    PMID: 23203801
  • Hui Guo, Yi Lv, Tao Tian et al,Downregulation of p57 accelerates the growth and invasion of hepatocellular carcinoma., Carcinogenesis, vol.32 no.12 pp.1897¨C1904 (2011),
    PMID: 22002319
  • Lijun Zhang, Jun Luo, Ping Wan et al,Regulation of cofilin phosphorylation and asymmetry in collective cell migration during morphogenesis., Development., 138:455-464 (2011),
    PMID: 21205790
  • Please let us know if you have published research using #11139 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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