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Histone H3.1(Phospho-Ser10) Antibody

11184
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  Catalog No. Package Size Price
11184-1 50ul $189.00
11184-2 100ul $279.00

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Description
  • Product Name Histone H3.1(Phospho-Ser10) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC IF
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of Histone H3.1 onlywhen phosphorylated at serine 10.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of serine 10 (R-K-S(p)-T-G) derived from Human Histone H3.1.
  • Target Name Histone H3.1
  • Modification Phospho
  • Alternative Names H3/b, H3FB
  • Accession No. Swiss-Prot: P68431
    NCBI Protein: NP_003521.2
  • SDS-PAGE MW 17
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 17kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images
  • Histone H3.1(Phospho-Ser10) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H3.1(Phospho-Ser10) Antibody #11184(left) or the same antibody preincubated with blocking peptide(right).

  • Histone H3.1(Phospho-Ser10) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from SK-BR-3 cells, treated with insulin and EGF, and pretreated with U0126 and LY294002 cells using Histone H3.1 (Phospho-Ser10) Antibody #11184.

  • Histone H3.1(Phospho-Ser10) Antibody - SAB | Signalway Antibody

    Immunofluorescence staining of methanol-fixed Hela cells using Histone H3.1 (Phospho-Ser10) Antibody #11184.

  • Histone H3.1(Phospho-Ser10) Antibody - SAB | Signalway Antibody

    Immunofluorescence staining of methanol-fixed Hela cells using Histone H3.1 (Phospho-Ser10) Antibody #11184.

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Dai J, et al. (2005) Genes Dev 19(4): 472-488.
Yih LH, et al. (2005) Carcinogenesis 26(1): 53-63.
 

  • Jiang et al,PKM2 Regulates Chromosome Segregation and Mitosis Progression of Tumor Cells, Molecular Cell, 53(1):75-87 (2014),
    PMID: 24316223
  • Lian-Qing Sun, Ying-Ying Chen, Xuan Wang et al,The protective effect of Alpha lipoic acid on Schwann cells exposed to constant or intermittent high glucose, Biochemical Pharmacology, 84 (2012) 961¨C973 (2012),
    PMID: 22796564
  • Yang W, Xia Y, Hawke D et al,PKM2 phosphorylates histone H3 and promotes gene transcription and tumorigenesis., Cell, 150(4):685-696 (2012),
    PMID: 22901803
  • Zenglin Liao, Jiajia Dong, Wei Wu et al,Resolvin D1 attenuates inflammation in lipopolysaccharide-induced acute lung injury through a process involving the PPAR¦Ã/NF-¦ÊB pathway, Respiratory Research , 13:110 (2012),
    PMID: 23199346
  • Please let us know if you have published research using #11184 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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