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VASP(Phospho-Ser157) Antibody

11214
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  Catalog No. Package Size Price
11214-1 50ul $189.00
11214-2 100ul $279.00

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Description
  • Product Name VASP(Phospho-Ser157) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC
  • Species Reactivity Hu Rt
  • Specificity The antibody detects endogenous level of VASP only when phosphorylated at serine 157.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of serine 157 (R-V-S(p)-N-A) derived from Human VASP.
  • Target Name VASP
  • Modification Phospho
  • Accession No. Swiss-Prot: P50552
    NCBI Protein: NP_003361.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 50kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images
  • VASP(Phospho-Ser157) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from C6 cells untreated or treated with PMA using VASP(Phospho-Ser157) Antibody #11214.

  • VASP(Phospho-Ser157) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human tonsil carcinoma tissue using VASP(Phospho-Ser157) Antibody #11214(left) or the same antibody preincubated with blocking peptide(right).

Background
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.

Zhao WM, et al. (2001) EMBO J 20(9): 2315-2325.
Millard TH, et al. (2005) EMBO J 24(2): 240-250.
K
    Please let us know if you have published research using #11214 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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