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p56Dok-2(Phospho-Tyr299) Antibody

11278
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  Catalog No. Package Size Price
11278-1 50ul $189.00
11278-2 100ul $279.00

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Description
  • Product Name p56Dok-2(Phospho-Tyr299) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC IF
  • Species Reactivity Hu
  • Specificity The antibody detects endogenous level of p56Dok-2 only when phosphorylated at tyrosine 299.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of tyrosine 299 (G-E-Y(p)-A-V) derived from Human p56Dok-2.
  • Target Name p56Dok-2
  • Modification Phospho
  • Alternative Names DOK2
  • Accession No. Swiss-Prot: O60496
    NCBI Protein: NP_003965.2
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 56kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images
  • p56Dok-2(Phospho-Tyr299) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from Jurkat cells untreated or treated with insulin using p56Dok-2(Phospho-Tyr299) Antibody #11278.

  • p56Dok-2(Phospho-Tyr299) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p56Dok-2(Phospho-Tyr299) Antibody #11278(left) or the same antibody preincubated with blocking peptide(right).

  • p56Dok-2(Phospho-Tyr299) Antibody - SAB | Signalway Antibody

    Immunofluorescence staining of methanol-fixed Hela cells using p56Dok-2(Phospho-Tyr299) Antibody #11278.

  • p56Dok-2(Phospho-Tyr299) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from JK cells, treated with G-CSF or calf intestinal phosphatase (CIP), using p56Dok-2 (Phospho-Tyr299) Antibody #11278.

Background

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation

Feng Cong, et,al. (1999) Mol. Cell. Biol ; 19: 8314 - 8325.
Serge Lemay, et,al. (2000) Mol. Cell. Biol ; 20: 2743 - 2754.
Ute Schaeper, et,al.(2000) J. Cell Biol ; 149: 1419.
Miyuki Honma, et,al. (2006) Genes Cells; 11: 143 - 151.

    Please let us know if you have published research using #11278 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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