Your Position: Home > Cytokines > Recombinant Human Epidermal Growth Factor ( rHu EGF )

Recombinant Human Epidermal Growth Factor ( rHu EGF )

70504
Review: comment rank 5 pdf
  Catalog No. Package Size Price
70504 500ug $235.00

Quantity:  Total:   
 

Availability: promotionYes

add promotion
Description
  • Product Name Recombinant Human Epidermal Growth Factor ( rHu EGF )
  • Brief Description Recombinant Protein
  • Host Species E.coli
  • Purification > 95 % by SDS-PAGE and HPLC analyses.
  • Species Reactivity Hu
  • Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb c 3T3 cells is less than 0.1 ng ml, corresponding to a specific activity of > 1.0 ⅹ 107 IU mg.
  • Endotoxin Less than 1 EU/��g of rHuEGF as determined by LAL method.
  • Target Name rHu EGF
  • Alternative Names Urogastrone, URG
  • Accession No. accession:P01133
    GeneID:Hs.419815.
  • Calculated MW Approximately 6.2 kDa, a singl
  • SDS-PAGE MW Sterile Filtered White lyophil
  • Target Sequence NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
  • Formulation Lyophilized from a 0.2 ��m filtered concentrated solution in PBS, pH 7.4.
  • Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio
  • Storage This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.
Images
  • Recombinant Human Epidermal Growth Factor ( rHu EGF ) - SAB | Signalway Antibody


Background
Epidermal Growth Factor (EGF) was originally discovered in crude preparations of nerve growth factor prepared from mouse submaxillary glands as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition, and stunting of growth when injected into newborn mice. Human EGF was isolated from urine based on its inhibitory effect on gastric secretion and named urogastrone, accordingly. EGF is prototypic of a family of growth factors that are derived from membrane-anchored precursors. All members of this family are characterized by the presence of at least one EGF structural unit (defined by the presence of a conserved 6 cysteine motif that forms three disulfide bonds) in their extracellular domain. EGF is initially synthesized as a 130 kDa precursor transmembrane protein containing 9 EGF units. The mature soluble EGF sequence corresponds to the EGF unit located proximal to the transmembrane domain. The membrane EGF precursor is capable of binding to the EGF receptor and was reported to be biologically active. Mature human EGF shares 70 % a.a. sequence identity with mature mouse and rat EGF.
Regerences
1. Chevalier RL, Goyal S, Thornhill BA. 1999. J Urol, 162: 1532-6. 2. Gehm BD, McAndrews JM, Jordan VC, et al. 2000. Mol Cell Endocrinol, 159: 53-62. 3. Yang H, Sun X, Wang Z, et al. 2003. J Membr Biol, 194: 47-58. 4. Cohen S. 2008. J Biol Chem, 283: 33793-7.
    Please let us know if you have published research using #70504 so that we can cite your reference.
Customer Reviews and Rankings
  • No comment
  • Total 0 results, divided into1 pages. First Prev Next Last

    My Review

    Username: Anonymous user
    E-mail:
    Ratings:
    Content:
    Verification code: captcha
     
    Protocol
    Browsing historyclear
    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
      SUBSCRIBE OUR E-MAIL
    • Inspiration, ideas and your feedback.
    FOLLOW US

    facebook twitter linkedin

    sab © 2017 Signalway Antibody LLC. All Rights Reserved.  E-mail: info@signalwayantibody.com