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Goat anti-Rabbit IgG Secondary Antibody HRP conjugated

L3012-2
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  Catalog No. Package Size Price
L3012-2 1ml $85.00

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Description
  • Product Name Goat anti-Rabbit IgG Secondary Antibody HRP conjugated
  • Brief Description Secondary Antibody
  • Clonality Polyclonal
  • Purification The antibody was obtained by affinity purified technique, which removes essentially all goat serum proteins, except the specific antibody for rabbit IgG. Goat anti-rabbit IgG was conjugated to peroxidase by means of a method described by Wilson MB and Nakane PK.
  • Applications ELISA WB IHC
  • Species Reactivity Rb
  • Specificity The antibody conjugate is specific for rabbit IgG. No cross-reactivity was observed with rabbit IgA, IgM proteins by ELISA.
  • Immunogen Type Protein
  • Immunogen Description The antiserum was developed in goat using the rabbit IgG as the immunogen.
  • Target Name Rabbit IgG
  • Formulation HRP-labeled Goat IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol.
  • Storage Store at -20 ℃/1 year
Application Details

ELISA : 1:5000~1:20000
Western blotting: 1:5000~1:10000
Immunohistochemistry: 1:1000~5000

Background

 Wilson MB and Nakane PK. In Immunofluorescence and Related

Staining Techniques, Elsevier/North Holland Biomedical Press,
Amsterdam, P215 (1978).
  • Saito S, Yamamoto H, Mukaisho K-i et al,Mechanisms Underlying Cancer Progression Caused by Ezrin Overexpression in Tongue Squamous Cell Carcinoma., PLoS ONE, 8(1): e54881. doi:10.1371/journal.pone.0054881 (2013),
    PMID: 23357878
  • YONG CHENG, LI-YOU AN, YU-GUO YUAN et al,Hybrid Expression Cassettes Consisting of a Milk Protein Promoter and a Cytomegalovirus Enhancer Significantly Increase Mammary-Specific Expression of Human Lactoferrin in Transgenic Mice., Molecular Reproduction & Development, 79(8):573¨C585 (2012),
    PMID: 22730016
  • Yudong Liu, Ying Su, Shenggang Sun et al,Tau Phosphorylation and m-Calpain Activation Mediate the Dexamethasone-Induced Inhibition on the Insulin-Stimulated Akt Phosphorylation. , PLoS ONE, 7(4): e35783. doi:10.1371/journal.pone.0035783 (2012),
    PMID: 22536436
  • Yonggen Jia, Xinxin Zhao, Jingru Zou et al,Trypanosoma evansi: Identification and characterization of a variant surface glycoprotein lacking cysteine residues in its C-terminal domain. , Experimental Parasitology, 127(1):100¨C106 (2011),
    PMID: 20599437
  • Ing-Chien Chen, za Chung-Ming Yu, za Yu-Ching Lee et al,Signal sequence as a determinant in expressing disulfide-stabilized single chain antibody variable fragments (sc-dsFv) against human VEGF, Mol. BioSyst., 6(7):1307¨C1315 (2010),
    PMID: 20424732
  • Please let us know if you have published research using #L3012-2 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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