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Histone H3 (Tri-Methyl-Lys27) Antibody

11582
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  Catalog No. Package Size Price
11582-1 50ul $189.00
11582-2 100ul $279.00

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Description
  • Product Name Histone H3 (Tri-Methyl-Lys27) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC IF
  • Species Reactivity Hu Ms Rt
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around Tri-Methylation site of lysine 27(A-R-K(tri-methyl)-S-A) derived from Human Histone H3.
  • Target Name Histone H3
  • Modification Methyl
  • Alternative Names H3/a, H3/c, H3/d, H3/f, H3/h
  • Accession No. Swiss-Prot#:P68431 NCBI Gene#:8351 NCBI Protein#:NP_003521.2
  • SDS-PAGE MW 17KD
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C/1 year
Application Details

Predicted MW: 17kd

Western Blot: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images
  • Histone H3 (Tri-Methyl-Lys27) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from HT29 and C6 cells using Histone H3 (Tri-Methyl-Lys27) Antibody #11582.

  • Histone H3 (Tri-Methyl-Lys27) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H3 (Tri-Methyl-Lys27) Antibody#11582.

  • Histone H3 (Tri-Methyl-Lys27) Antibody - SAB | Signalway Antibody

    Immunofluorescence staining of methanol-fixed MEF cells using Histone H3 (Tri-Methyl-Lys27) Antibody #11582.

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

    Please let us know if you have published research using #11582 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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