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V5-Tag Mouse Monoclonal Antibody

T605
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  Catalog No. Package Size Price
T605 100ul $279.00

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Description
  • Product Name V5-Tag Mouse Monoclonal Antibody
  • Host Species Mouse
  • Clonality Monoclonal
  • Isotype IgG1
  • Purification Affinity-chromatography
  • Applications WB IHC IF IP
  • Specificity V5-tag mouse monoclonal antibody detects endogenous levels of internal, C-terminal, and N-terminal V5- tagged proteins.
  • Immunogen Type Peptide-KLH
  • Immunogen Description A synthetic peptide GKPIPNPLLGLDST coupled to KLH.
  • Target Name V5-Tag
  • SDS-PAGE MW N/A
  • Concentration 1.0mg/ml
  • Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20 °C.Stable for 12 months from date of receipt
Application Details

Western blotting      1:3000-1:10000
Immunohistochemistry  1:200

Images
  • V5-Tag Mouse Monoclonal Antibody - SAB | Signalway Antibody

    All lanes : V5-tag mouse monoclonal antibody A: HEK 293 cell lysate transfected without V5-tag protein B: HEK 293 cell lysate transfected with V5 -tag protein C: IP (HEK 293 cell without transfected + anti- V5 mAb 4℃ overnight) D: IP (PBS + anti- V5 mAb 4℃ overnight) E: IP (HEK293 transfected+protein G+anti- V5 mAb 4℃ overnight) F: IP (HEK293 transfected+RIPA+Protein G 4℃ overnight) G: Multi Tag Recombinant protein (expressed in E.coli) TP=Target Protein HC=Heavy chain LC=Light Chain

  • V5-Tag Mouse Monoclonal Antibody - SAB | Signalway Antibody

    IF analysis of 293 cells transfected with a V5-tag protein, using anti-V5Tag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-V5)

  • V5-Tag Mouse Monoclonal Antibody - SAB | Signalway Antibody

    IP antibody use:5ug V5 Mouse IgG1 per ml Lysate,WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with V5-tag fusion protein 3、IP(untransfted 293+anti-V5 mAb+Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 5、IP (transfected 293+anti-V5 mAb+ Protein G agarose) 6、IP (transfected 293+Protein G) 7、Recombinant protein (E.coli)

Background

Background: The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).
 

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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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