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PCNA Mouse Monoclonal Antibody

21801
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  Catalog No. Package Size Price
21801 100ul $279.00

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Description
  • Product Name PCNA Mouse Monoclonal Antibody
  • Host Species Mouse
  • Clonality Monoclonal
  • Purification Affinity-chromatography
  • Applications WB IHC
  • Species Reactivity Hu Ms Rt
  • Immunogen Type Peptide-KLH
  • Immunogen Description Mouse monoclonal antibody is prepared by immunizing a synthetic peptide.
  • Target Name PCNA
  • Alternative Names Cyclin; DNA polymerase delta auxiliary protein; MGC8367; PCNA; Proliferating cell nuclear antigen.
  • SDS-PAGE MW 29KD
  • Concentration 1.0mg/ml
  • Formulation Mouse IgG1 in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C/1 year
Application Details

 WB: 1:3000~1:10000   
 IHC: 1:200

 

Images
  • PCNA Mouse Monoclonal Antibody - SAB | Signalway Antibody

    Western blot analysis of Hela (1), Rat brain (2), NIH 3T3 (3), 293T(4) with PCNA mouse mAb(12D10) diluted at 1:5000.

Background

 PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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