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PI3-kinase p85- alpha (Phospho-Tyr607) Antibody

12057
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  Catalog No. Package Size Price
12057 100ul $279.00

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Description
  • Product Name PI3-kinase p85- alpha (Phospho-Tyr607) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of PI3-kinase p85- alpha only when phosphorylated at Tyrosine 607.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of Tyrosine 607 (D-Q-Y(p)-S-L) derived from Human PI3-kinase p85-alpha.
  • Target Name PI3-kinase p85- alpha
  • Modification Phospho
  • Alternative Names p85, AGM7, GRB1, p85-ALPHA
  • Accession No. Swiss-Prot#: P27986;
    NCBI Gene#: 5295;
    NCBI Protein#: NP_001229395.1
  • SDS-PAGE MW 80kd
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20˚C/1 year
Application Details

Predicted MW: 80kd
Western blotting: 1:500~1:1000

Images
  • PI3-kinase p85- alpha (Phospho-Tyr607) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from Rat liver tissue using PI3-kinase p85- alpha (Phospho-Tyr607) Antibody #12057.The lane on the right is treated with the antigen-specific peptide.

  • PI3-kinase p85- alpha (Phospho-Tyr607) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from Hela cell treated with H2O2 using PI3-kinase p85- alpha (Phospho-Tyr607) Antibody #12057

  • PI3-kinase p85- alpha (Phospho-Tyr607) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human brain tissue tissue usingPI3-kinase p85- alpha (Phospho-Tyr607) Antibody #12057.

  • PI3-kinase p85- alpha (Phospho-Tyr607) Antibody - SAB | Signalway Antibody

    Lane 1 : N-peptide blocked MCF7 cell extract; Lane 2 : MCF7 cell extract; Lane 3 : P-peptide blocked MCF7 cell extract; Lane 4 : N-peptide blocked Mouse brain tissue; Lane 5 : Mouse brain tissue; Lane 6 : P-peptide blocked Mouse brain tissue; Lane 7 : N-peptide blocked Rat brain tissue; Lane 8 : Rat brain tissue; Lane 9 : P-peptide blocked Rat brain tissue; Lysates/proteins at 40 µg per lane. Predicted band size : 80 kDa Observed band size : 80 kDa

Background

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling.

    Please let us know if you have published research using #12057 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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