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Bub3 (Phospho-Tyr207) Antibody

11586
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  Catalog No. Package Size Price
11586-1 50ul $189.00
11586-2 100ul $279.00

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Description
  • Product Name Bub3 (Phospho-Tyr207) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of Bub3 only when phosphorylated at Tyrosine 207.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of Tyrosine 207 (V-E-Y(p)-L-D) derived from Human Bub3.
  • Target Name Bub3
  • Modification Phospho
  • Alternative Names Mitotic checkpoint protein BUB3, Hbub3, BUB3L, BUB3 budding uninhibited by benzimidazoles 3 homolog, budding uninhibited by benomyl
  • Accession No. Swiss-Prot#: O43684 NCBI Protein#: NP_001007794.1
  • SDS-PAGE MW 40KD
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C/1 year
Application Details

 Western blotting: 1:1000

Images
  • Bub3 (Phospho-Tyr207) Antibody - SAB | Signalway Antibody

    Western blot analysis of Bub3 (Phospho-Tyr207)Antibody #11586 (arrow indicated) in U87 (A) and U87 EGFRvIII (B) cells in Mitosis.

Background
Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
 
 
Seeley T.W., Wang L., Zhen J.Y.Biochem. Biophys. Res. Commun. 257:589-595(1999)
Tang Z., Shu H., Oncel D., Chen S., Yu H.Mol. Cell 16:387-397(2004)
Logarinho E., Resende T., Torres C., Bousbaa H.Mol. Biol. Cell 19:1798-1813(2008)
 
    Please let us know if you have published research using #11586 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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