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PKM1 Antibody

21577
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  Catalog No. Package Size Price
21577-1 50ul $189.00
21577-2 100ul $279.00

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Description
  • Product Name PKM1 Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Applications WB IF
  • Species Reactivity Hu Ms
  • Specificity The antibody detects endogenous levels of total PKM1 protein.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around aa. 399~403(V-R-A-S-S) derived from Human PKM1.
  • Target Name PKM1
  • Alternative Names PKM; PK3; OIP3; PK2;
  • Accession No. Swiss-Prot: P14618
    NCBI Protein: NP_872270.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 60kd
Western blotting: 1:1000

Images
  • PKM1 Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from U87 cells using PKM1 Antibody #21577.

  • PKM1 Antibody - SAB | Signalway Antibody

    Immunofluorescence staining of methanol-fixed MEF cells using PKM1 Antibody #21577.

Background

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Christofk, H.R. et al. (2008) Nature 452, 230-3.
Mazurek, S. et al. (2005) Semin Cancer Biol 15, 300-8.
Dombrauckas, J.D. et al. (2005) Biochemistry 44, 9417-29.
Hitosugi, T. et al. (2009) Sci Signal 2, ra73.

    Please let us know if you have published research using #21577 so that we can cite your reference.
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    Protocol
    Browsing historyclear
    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
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