Your Position: Home > Phospho-specific Antibodies > PTEN(Phospho-Ser380/Thr382/Thr383) Antibody

PTEN(Phospho-Ser380/Thr382/Thr383) Antibody

11056
Review: comment rank 5 pdf
  Catalog No. Package Size Price
11056-1 50ul $189.00
11056-2 100ul $279.00

Quantity:  Total:   
 

Availability: promotionYes

add promotion
Description
  • Product Name PTEN(Phospho-Ser380/Thr382/Thr383) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC IF
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of PTEN only when phosphorylated at serine 380 and threonine 382/383.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of threonine 380/382/383 (R-Y-S(p)-D-T(p)-T(p)-D-S) derived from Human PTEN.
  • Target Name PTEN
  • Modification Phospho
  • Alternative Names MMAC1; Mutated in multiple advanced cancers 1; Protein-tyrosine phosphatase PTEN; TEP1;
  • Accession No. Swiss-Prot: P60484
    NCBI Protein: NP_000305.3
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 54kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images
  • PTEN(Phospho-Ser380/Thr382/Thr383) Antibody - SAB | Signalway Antibody

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PTEN(Phospho-Ser380/Thr382/Thr383) Antibody #11056(left) or the same antibody preincubated with blocking peptide(right).

  • PTEN(Phospho-Ser380/Thr382/Thr383) Antibody - SAB | Signalway Antibody

    Immunofluorescence staining of methanol-fixed Hela cells using PTEN(Phospho-Ser380/Thr382/Thr383) Antibody #11056.

  • PTEN(Phospho-Ser380/Thr382/Thr383) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody #11056.

  • PTEN(Phospho-Ser380/Thr382/Thr383) Antibody - SAB | Signalway Antibody

    Western blot analysis of extracts from Hela and C6 cells,treated with EGF or PMA, using PTEN(Phospho-Ser380/Thr382/Thr383) Antibody #11056.

  • PTEN(Phospho-Ser380/Thr382/Thr383) Antibody - SAB | Signalway Antibody

    Immunofluorescence staining of methanol-fixed MEFcells using PTEN (Phospho-Ser380/Thr382/Thr383)Antibody #11056.

Background

Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue.

Al-Khouri AM, et al. (2005) J Biol Chem. 280(42):35195-35202.
Torres J, et al. (2001) J Biol Chem. 276(2): 993-998.
Vazquez F, et al. (2000) Mol Cell Biol. 20(14): 5010-5018.

    Please let us know if you have published research using #11056 so that we can cite your reference.
Customer Reviews and Rankings
  • No comment
  • Total 0 results, divided into1 pages. First Prev Next Last

    My Review

    Username: Anonymous user
    E-mail:
    Ratings:
    Content:
    Verification code: captcha
     
    Protocol
    Browsing historyclear
    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  
      SUBSCRIBE OUR E-MAIL
    • Inspiration, ideas and your feedback.
    FOLLOW US

    facebook twitter linkedin

    sab © 2017 Signalway Antibody LLC. All Rights Reserved.  E-mail: info@signalwayantibody.com